Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis

Abd Al‑Bar Al‑Farha, Kiro Petrovski, Razi Jozani, Andrew Hoare and Farhid Hemmatzadeh - BMC Research Notes

Type: Research Paper
Knowledge level: Advanced

Farm Table says:

A new, molecular method is reported for the detection and differentiation of mycoplasmas causing mastitis in an SA dairy herd. The new method is promising, and may be useful in the future.

What is the problem?

Mycoplasma mastitis is a significant problem in the dairy industry, with many subspecies existing. The current methods for the differentiation and detection of these for treatment is difficult, expensive and time consuming. The authors, from the University of Adelaide, report the development of a molecular method for the detection and differentiation of the different mycoplasmas for mastitis on a dairy farm in South Australia.

What did the research involve?

Using 5 mycoplasma isolates from a commercial dairy farm in South Australia, the authors looked to validate a molecular method, polymerase chain reaction high resolution melt (PCR-HRM), to genotype mycoplasma infections. This method involves amplifying DNA specific to different genotypes, and subsequently differentiating based on the temperature the different genotype DNA melts at.

What were the key findings?

The researchers were successfully able to identify the different types of mycoplasmas from the field sample isolates. This is reported as a cheaper and more sensitive method to the traditional methods.

Final Comment

The authors were successful at correctly identifying the 5 isolates, and state this may be a higher throughput, sensitive, and cheaper method than what is previously used. However, it is not able to identify different mycoplasmas from mixed infections.

2018 - Australia - Abd Al‑Bar Al‑Farha, Kiro Petrovski, Razi Jozani, Andrew Hoare and Farhid Hemmatzadeh - BMC Research Notes
Read ArticleSave For Later

Related Resources